Conjugation

Part:BBa_K1053124:Design

Designed by: Yasuhito Ito   Group: iGEM13_Tokyo-NoKoGen   (2013-09-10)

Split EYFP C-terminal domain fused with λN peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

λN peptide was fused to the split EYFP C-terminal domain via a flexible 10 residues linker (Gly-Ser-Ser-Gly-Ser-Ser-Gly-Ser-Gly-Ser)

Source

the split EYFP C-terminal domain is deriverd from BBa_E0030.

λN peptide is synthesis.

References

Hung-Wei Yiu, Vadim V. Demidov, Paul Toran, Charles R. Cantor and Natalia E. Broude, "RNA Detection in Live Bacterial Cells Using Fluorescent Protein Complementation Triggered by Interaction of Two RNA Aptamers with Two RNA-Binding Peptides" Pharmaceuticals 2011, 4, 494-508; doi:10.3390/ph4030494